CHMP4B Rekombinanter Antikörper

CHMP4B Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

2N22

Kat-Nr. : 81691-2-RR

Synonyme

hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B, C20orf178

---

• 

  WB analysis using 81691-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81691-2-RR (CHMP4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 81691-2-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CHMP4B Recombinant antibody (81691-2-RR, Clone:2N22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 81691-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81691-2-RR against Human CHMP4B were performed. The affinity constant is 4.59 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, C2C12-Zellen, HeLa-Zellen, HuH-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81691-2-RR bindet in WB, FC (Intra), ELISA CHMP4B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CHMP4B fusion protein Ag4544
• Vollständiger Name: chromatin modifying protein 4B
• Berechnetes Molekulargewicht: 224 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC033859
• Gene symbol: CHMP4B
• Gene ID (NCBI): 128866
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).