CHMP6 Polyklonaler Antikörper

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• KD/KO Validated

CHMP6 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 16278-1-AP

Synonyme

Vps20, Vacuolar protein sorting-associated protein 20, hVps20, Chromatin-modifying protein 6, chromatin modifying protein 6

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  WB analysis of mouse testis using 16278-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16278-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 16278-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 16278-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 16278-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 16278-1-AP

  IP result of anti-CHMP6 (IP:16278-1-AP, 4ug; Detection:16278-1-AP 1:500) with mouse testis tissue lysate 2400ug.

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  IHC staining of human colon cancer using 16278-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 16278-1-AP (CHMP6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 16278-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16278-1-AP (CHMP6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, A375-Zellen, A431-Zellen, Jurkat-Zellen, U-937-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, human colon cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

16278-1-AP bindet in WB, IHC, IF, IP, ELISA CHMP6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHMP6 fusion protein Ag9357
• Vollständiger Name: chromatin modifying protein 6
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC010108
• Gene symbol: CHMP6
• Gene ID (NCBI): 79643
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
canine	WB	Curr Biol; A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.; Authors - Eva P Karasmanis
		J Cell Biol; ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes.; Authors - Jorge Larios
human	WB	J Virol; A non-envelopment role for the ESCRT-III complex during HCMV infection.; Authors - Nicholas T Streck
human	WB	J Virol; Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread.; Authors - Nicholas T Streck
human	WB,IF	Nat Commun; Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy; Authors - Takashi Nozawa ; KD Validated