CHRNB1 Polyklonaler Antikörper

CHRNB1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11553-1-AP

Synonyme

ACHRB, CHRNB, CHRNB1, CMS1D, CMS2A, SCCMS

---

Galerie der Validierungsdaten

• 

  WB analysis of human liver using 11553-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11553-1-AP (CHRNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11553-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11553-1-AP (CHRNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11553-1-AP

  IP Result of anti-CHRNB1 (IP:11553-1-AP, 4ug; Detection:11553-1-AP 1:300) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 11553-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11553-1-AP (CHRNB1 antibody) at dilution of 1:50 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

11553-1-AP bindet in WB, IP, IHC, ELISA CHRNB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHRNB1 fusion protein Ag2108
• Vollständiger Name: cholinergic receptor, nicotinic, beta 1 (muscle)
• Berechnetes Molekulargewicht: 501 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: BC023553
• Gene symbol: CHRNB1
• Gene ID (NCBI): 1140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acetylcholine receptors (AChRs) are integral membrane proteins that respond to the binding of acetylcholine (ACh), a neurotransmitter synthesized, stored and finally released by cholinergic neurons. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The muscle acetylcholine receptor is composed of five subunits: two alpha subunits and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) subunits. CHRNB1 gene encodes acetylcholine receptor subunit beta (ACHRB). Mutations in this gene are associated with slow-channel congenital myasthenic syndrome.

Publikationen

Species	Application	Title
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo
mouse	WB	Genes Immun; Plasticity of the murine spleen T-cell cholinergic receptors and their role in in vitro differentiation of na?ve CD4 T cells toward the Th1, Th2 and Th17 lineages.; Authors - Qian J J
mouse	WB	Biochem Biophys Res Commun; Association of a newly identified lncRNA LNC_000280 with the formation of acetylcholine receptor clusters in vitro.; Authors - Jin Deng
mouse	WB	Mol Ther Nucleic Acids; MiR-142a-3p: A novel ACh receptor transcriptional regulator in association with peripheral nerve injury; Authors - Zhidan Qi