CHURC1 Polyklonaler Antikörper

CHURC1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12247-1-AP

Synonyme

C14orf52, chch, CHURC1, churchill domain containing 1, My015, Protein Churchill

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Galerie der Validierungsdaten

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  WB analysis of mouse skin using 12247-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12247-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12247-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12247-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 12247-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 12247-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12247-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 12247-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 12247-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 12247-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, HeLa-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Gliomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12247-1-AP bindet in WB, IHC,ELISA CHURC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHURC1 fusion protein Ag2892
• Vollständiger Name: churchill domain containing 1
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC020550
• Gene symbol: CHURC1
• Gene ID (NCBI): 91612
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHURC1 (churchill domain containing 1), also known as C14orf52 or chch. The function of CHURC1 has not been widely studied, and is yet to be fully elucidated. It may be a transcriptional activator that mediates FGF signaling during neural development.The MW of this protein is 13 kDa, and Catalog#12247-1-AP specially recognises the 13 kDa protein.