CILP2 Polyklonaler Antikörper

CILP2 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11813-1-AP

Synonyme

CILP 2, CILP2, CLIP 2

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 11813-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11813-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11813-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11813-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11813-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 11813-1-AP

  Immunofluorescent analysis of HEK-293 cells using 11813-1-AP (CILP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11813-1-AP bindet in WB, IF, ELISA CILP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CILP2 fusion protein Ag2366
• Vollständiger Name: cartilage intermediate layer protein 2
• Berechnetes Molekulargewicht: 126 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC034926
• Gene symbol: CILP2
• Gene ID (NCBI): 148113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CILP2, also named as Cartilage intermediate layer protein 2, is a 1156 amino acid protein, which is expressed in articular chondrocytes but not in knee meniscal cartilage cells. CILP2 localizes to the intermediate to deep zone of articular cartilage. CILP2 may play a role in cartilage scaffolding. Given the full-length molecular mass of CILP2 is 125 kDa, but the most CILP2 protein in articular cartilage is cleaved,mostly likely at the furin cleavage site. CILP2 protein is detected as a major band on SDS-page migrating at about 90 kDa. (PMID: 21880736)

Publikationen

Species	Application	Title
mouse	WB	Cell Rep; Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development; Authors - Mengtian Zhang