CIN85 Polyklonaler Antikörper

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CIN85 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 12132-1-AP

Synonyme

SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3

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  WB analysis using 12132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 12132-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12132-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12132-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 12132-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, fetales humanes Hirngewebe, Jurkat-Zellen, NIH/3T3-Zellen, Ramos-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12132-1-AP bindet in WB, IHC, IF, ELISA CIN85 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CIN85 fusion protein Ag2777
• Vollständiger Name: SH3-domain kinase binding protein 1
• Berechnetes Molekulargewicht: 665 aa, 73 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC015806
• Gene symbol: SH3KBP1/CIN85
• Gene ID (NCBI): 30011
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

Publikationen

Species	Application	Title
human	WB, IF	J Cell Biol; CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface.; Authors - Ihor Yakymovych
human	WB	Biol Cell; ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.; Authors - Yoon Hye-Young HY ; KD Validated
human	IHC	Arch Gynecol Obstet; Significance of PTPRZ1 and CIN85 expression in cervical carcinoma.; Authors - Ma Yaxi Y