CISD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CISD2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 13318-1-AP

Synonyme

CDGSH iron sulfur domain 2, CDGSH2, CISD2, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2

---

Galerie der Validierungsdaten

• 

  WB analysis using 13318-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13318-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13318-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13318-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13318-1-AP

  IP Result of anti-CISD2 (IP:13318-1-AP, 3ug; Detection:13318-1-AP 1:700) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 13318-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13318-1-AP (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13318-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13318-1-AP (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13318-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CISD2 antibody (13318-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13318-1-AP

  Immunofluorescent analysis of HepG2 cells using 13318-1-AP (CISD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HepG2 using 13318-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug CISD2 antibody (13318-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, humanes Hirngewebe, Mausherzgewebe, Maushirngewebe, Rattennierengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 20 publications below
• IHC: See 10 publications below
• IF: See 4 publications below

Produktinformation

13318-1-AP bindet in WB, IP, IHC, IF, FC, ELISA CISD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, drosophila, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CISD2 fusion protein Ag4172
• Vollständiger Name: CDGSH iron sulfur domain 2
• Berechnetes Molekulargewicht: 135 aa, 15 kDa
• Beobachtetes Molekulargewicht: 13-15 kDa
• GenBank-Zugangsnummer: BC032300
• Gene symbol: CISD2
• Gene ID (NCBI): 493856
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.

Publikationen

Species	Application	Title
human, mouse, rat	WB	Proc Natl Acad Sci U S A; A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome.; Authors - Simin Lu ; KD Validated
	WB	Elife; Sampling the conformational space of the catalytic subunit of human γ-secretase.; Authors - Xiao-Chen Bai
human	WB	EMBO Rep; Chemical targeting of NEET proteins reveals their function in mitochondrial morphodynamics.; Authors - Diana Molino ; KD Validated
human	WB,IHC	Oncotarget; Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.; Authors - Lan Wang ; KD Validated
mouse	WB,IHC	FASEB J; A young testicular microenvironment protects Leydig cells against age-related dysfunction in a mouse model of premature aging.; Authors - Michael Curley ; KO Validated
human	WB,IHC	Oncotarget; A novel prognostic score model incorporating CDGSH Iron Sulfur Domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.; Authors - Lin Yang

Verwandte Produkte

CISD1 Polyclonal antibody

16006-1-AP

WFS1 Polyclonal antibody

11558-1-AP

human BCL2 Polyclonal antibody

12789-1-AP

AKAP10 Polyclonal antibody

12356-1-AP

Calnexin Polyclonal antibody

10427-2-AP

OPA3 Polyclonal antibody

15638-1-AP