Cytokeratin 7 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Cytokeratin 7 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

CloneNo.

2E1G5

Rezensionen (1)

Kat-Nr. : 66483-1-Ig

Synonyme

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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  WB analysis using 66483-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66483-1-Ig

  WB result of CK7 antibody (66483-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CK7 transfected HeLa cells.

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  WB analysis of A431 using 66483-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 66483-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66483-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66483-1-Ig (CK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66483-1-Ig (CK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66483-1-Ig (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human prostate cancer using 66483-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 7 antibody (66483-1-Ig, Clone: 2E1G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human prostate cancer using 66483-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 7 antibody (66483-1-Ig, Clone: 2E1G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen, PC-3-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Ovarialkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Prostatakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:40000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 7 publications below

Produktinformation

66483-1-Ig bindet in WB, IHC, IF-P, ELISA Cytokeratin 7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 7 fusion protein Ag7895
• Vollständiger Name: keratin 7
• Berechnetes Molekulargewicht: 469 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC002700
• Gene symbol: Cytokeratin 7
• Gene ID (NCBI): 3855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

Publikationen

Species	Application	Title
human	IHC,IF	Oxid Med Cell Longev; Deletion of ACLY Disrupts Histone Acetylation and IL-10 Secretion in Trophoblasts, Which Inhibits M2 Polarization of Macrophages: A Possible Role in Recurrent Spontaneous Abortion.; Authors - Xin Chen
human	IHC	Front Oncol; Significance of mouse xenograft tumor model using patient-derived cancer organoids for clinical drug development; Authors - Hisataka Ogawa
human	WB	Sci Rep; Long-term expansion of basal cells and the novel differentiation methods identify mechanisms for switching Claudin expression in normal epithelia; Authors - Akihito Inoko
human	IF	Life Sci; Cyclophilin A inhibits trophoblast migration and invasion in vitro and vivo through p38/ERK/JNK pathways and causes features of preeclampsia in mice.; Authors - Haoyue Hu
human	IHC	Placenta; MiR-187 regulates the proliferation, migration and invasion of human trophoblast cells by repressing BCL6-mediated activation of PI3K/AKT signaling.; Authors - Xin Chen
human	IF	Gene; Comprehensive analysis of placental gene-expression profiles and identification of EGFR-mediated autophagy and ferroptosis suppression in intrahepatic cholestasis of pregnancy.; Authors - Yan Fang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Santhosh (Verified Customer) (04-15-2019)

Human breast tumor FFPE tissue. HIER using citrate buffer. 1:200

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200

Cytokeratin 7 Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66483-1-Ig)