CKAP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CKAP2 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 25486-1-AP

Synonyme

CKAP2, CTCL tumor antigen se20 10, LB1, se20 10, TMAP

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 25486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25486-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25486-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 25486-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 25486-1-AP

  Immunofluorescent analysis of HeLa cells using 25486-1-AP (CKAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, PC-3-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

25486-1-AP bindet in WB, IF, ELISA CKAP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CKAP2 fusion protein Ag21901
• Vollständiger Name: cytoskeleton associated protein 2
• Berechnetes Molekulargewicht: 683 aa, 77 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC136332
• Gene symbol: CKAP2
• Gene ID (NCBI): 26586
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oncol Rep; CKAP2 expression is associated with glioma tumor growth and acts as a prognostic factor in high‑grade glioma.; Authors - Kuanyu Wang ; KD Validated
human	WB,IF	Cancers (Basel); Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer; Authors - Alexsandro dos Santos

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azriel (Verified Customer) (12-02-2019)

Worked great for IF of HT-1080 cells at a 1/400 dilution incubation overnight

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: HT-1080 human fibrosarcoma cell line