CKAP2L Polyklonaler Antikörper

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• KD/KO Validated

CKAP2L Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17143-1-AP

Synonyme

Cytoskeleton-associated protein 2-like, Radial fiber and mitotic spindle protein, Radmis

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  WB analysis using 17143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17143-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17143-1-AP

  IP result of anti-CKAP2L (IP:17143-1-AP, 4ug; Detection:17143-1-AP 1:2000) with Jurkat cells lysate 1560 ug.

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  IHC staining of human colon cancer using 17143-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 17143-1-AP (CKAP2L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: human colon cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

17143-1-AP bindet in WB, IHC, IP, ELISA CKAP2L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CKAP2L fusion protein Ag10909
• Vollständiger Name: cytoskeleton associated protein 2-like
• Berechnetes Molekulargewicht: 745 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC036217
• Gene symbol: CKAP2L
• Gene ID (NCBI): 150468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKAP2L is a microtubule-binding protein that is crucial for the formation of the mitotic spindle and the cell cycle progression of neural progenitor cells. Mutations in CKAP2L have been associated with spindle organization defects, including mitotic spindle defects, lagging chromosomes, and chromatin bridges. There is evidence that mutations in this gene are associated with Filippi syndrome, characterized by growth defects, microcephaly, intellectual disability, facial feature defects, and syndactyly(PMID: 25439729).

Publikationen

Species	Application	Title
mouse	WB	Int J Clin Exp Pathol; Oncogenic and prognostic role of CKAP2L in hepatocellular carcinoma.; Authors - Penghui Wang ; KD Validated
human	WB,IHC	FASEB J; IGF2BP2 promotes ovarian cancer growth and metastasis by upregulating CKAP2L protein expression in an m6 A-dependent manner; Authors - Yaqian Shi