CKAP4 Polyklonaler Antikörper

CKAP4 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Rezensionen (1)

Kat-Nr. : 16686-1-AP

Synonyme

63 kDa membrane protein, CKAP4, CLIMP 63, ERGIC 63, p63

---

Galerie der Validierungsdaten

• 

  WB analysis using 16686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 16686-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16686-1-AP

  IP Result of anti-CKAP4 (IP:16686-1-AP, 4ug; Detection:16686-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human kidney using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16686-1-AP (CKAP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16686-1-AP (CKAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 16686-1-AP (CKAP4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 16686-1-AP (CKAP4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16686-1-AP

  Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody 16686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 16686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP4 antibody (16686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP4 antibody (16686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen, Rattennierengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 12 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

16686-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA CKAP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CKAP4 fusion protein Ag10019
• Vollständiger Name: cytoskeleton-associated protein 4
• Berechnetes Molekulargewicht: 602 aa, 66 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC082972
• Gene symbol: CKAP4
• Gene ID (NCBI): 10970
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

Publikationen

Species	Application	Title
mouse	WB	Nature; Regulation of endoplasmic reticulum turnover by selective autophagy.; Authors - Aliaksandr Khaminets
rat	WB	Cell Metab; Palmitoylation couples insulin hypersecretion with β cell failure in diabetes; Authors - Guifang Dong
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.; Authors - Michael L van de Weijer
mouse	IF	EMBO J; ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport.; Authors - Ilaria Fregno
	IF	EMBO J; MiT/TFE factors control ER-phagy via transcriptional regulation of FAM134B.; Authors - Laura Cinque

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

WEI (Verified Customer) (06-28-2020)

Single strong band at correct size.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Heart

Verwandte Produkte

tPA Polyclonal antibody

10147-1-AP

RPN2 Polyclonal antibody

10576-1-AP

RPN1 Polyclonal antibody

12894-1-AP

TUSC3 Polyclonal antibody

16039-1-AP

DDOST Polyclonal antibody

14916-1-AP

STT3A Polyclonal antibody

12034-1-AP