CKB-Specific Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

CKB-Specific Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240418D2

Kat-Nr. : 83522-5-RR

Synonyme

CKB, KCRB, 240418D2, B CK, B-CK

---

• 

  WB analysis using 83522-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83522-5-RR (CKB-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HAP1 using 83522-5-RR

  WB result of sg-CKB-Specific antibody (83522-5-RR; 1:10000; room temperature for 1.5 hours) with wild-type and sg-CKB-Specific transfected HAP1 cells.

• 

  IHC staining of human colon cancer using 83522-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83522-5-RR (CKB-Specific antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 83522-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CKB-Specific Recombinant Antibody (83522-5-RR, Clone:240418D2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83522-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83522-5-RR against Human CKB were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:100-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83522-5-RR bindet in WB, IHC, FC (Intra), ELISA CKB-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CKB-Specific fusion protein Ag7285
• Vollständiger Name: creatine kinase, brain
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC001190
• Gene symbol: CKB
• Gene ID (NCBI): 1152
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.