CKM-Specific Polyklonaler Antikörper

CKM-Specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 18712-1-AP

Synonyme

CKM, Creatine phosphokinase M-type, Creatine kinase M-type, CPK-M, CKMM



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Mausherzgewebe, Maus-Dünndarmgewebe
Erfolgreiche Detektion in IHCMaus-Skelettmuskelgewebe, Rattenherzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 6 publications below
IFSee 1 publications below

Produktinformation

18712-1-AP bindet in WB, IHC, IF, ELISA CKM-Specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte ReaktivitätMaus, Ratte, Bakterium
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name creatine kinase, muscle
Berechnetes Molekulargewicht 43 kDa
Beobachtetes Molekulargewicht 43 kDa
GenBank-ZugangsnummerBC007462
Gene symbol CKM
Gene ID (NCBI) 1158
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CKM, also named CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in the heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain, and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB exists in the myocardium, and BB exists in many tissues, especially the brain. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme. This oxidized form of creatine kinase was rapidly reconverted to the 42 kDa species by the addition of reducing agents concomitant with restoration of activity. (PMID: 10827185). This antibody is specific to CKM.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CKM-Specific antibody 18712-1-APProtokoll herunterladen
IHC protocol for CKM-Specific antibody 18712-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
mouseWB

Cell Prolif

KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.

Authors - Jing Hao
mouseWB

Toxicology

Pb inhibited C2C12 myoblast differentiation by regulating HDAC2

Authors - Xiaozhen Gu
BacterialWB

Mol Pharmacol

Naturally Occurring Mutations to Muscle-Type Creatine Kinase Impact Its Canonical and Pharmacological Activities in a Substrate-Dependent Manner In Vitro.

Authors - Eric P Mosher
mouseWB

BMC Musculoskelet Disord

Ang-(1-7) protects skeletal muscle function in aged mice.

Authors - Ying Li
mouse,ratWB,IF

Phytomedicine

Ligustilide covalently binds to Cys254 of the creatine kinase, M-type protein, ameliorating acute myocardial ischemia by enhancing the creatine phosphate level

Authors - Kaixue Zhang