CLASP1 Polyklonaler Antikörper

CLASP1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC,ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 20803-1-AP

Synonyme

CLASP1, CLIP associating protein 1, hOrbit1, KIAA0622, MAST1, Multiple asters homolog 1, Protein Orbit homolog 1

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Galerie der Validierungsdaten

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  WB analysis using 20803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20803-1-AP (CLASP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20803-1-AP

  IP Result of anti-CLASP1 (IP:20803-1-AP, 4ug; Detection:20803-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human testis using 20803-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20803-1-AP (CLASP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 20803-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20803-1-AP (CLASP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20803-1-AP bindet in WB, IP, IHC,ELISA CLASP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLASP1 fusion protein Ag14724
• Vollständiger Name: cytoplasmic linker associated protein 1
• Berechnetes Molekulargewicht: 1538 aa, 169 kDa
• Beobachtetes Molekulargewicht: 162 kDa
• GenBank-Zugangsnummer: BC112940
• Gene symbol: CLASP1
• Gene ID (NCBI): 23332
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLASP1 is a member of CLASP family. Members of the CLASP family of proteins are involved in multiple MT-dependent processes, including Golgi complex organization and trafficking, kinetochore fiber dynamics and flux, mitotic spindle assembly, and the stabilization of MT plus ends near the cortex. CLASP proteins regulate MT dynamics by promoting MT rescue and suppressing MT catastrophe. Recent work showed that CLASP1 activity is required for proper positioning of the mitotic spindle in both the C. elegans embryo and mammalian cells. Recent work has also identified roles CLASP1 in the regulation of spindle positioning.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (11-05-2023)

Excellent antibody for detection of CLASP1 via Western Blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVEC

CLASP1 Antibody Western Blot validation (1:1000 dilution) in HUVEC (Cat no:20803-1-AP)