Claudin 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Claudin 1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte und mehr (4)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

212

Rezensionen (2)

Kat-Nr. : 13050-1-AP

Synonyme

claudin 1, CLD1, CLDN1, ILVASC, SEMP1

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Galerie der Validierungsdaten

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  WB analysis using 13050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13050-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP (CLDN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13050-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 13050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Claudin 1 antibody (13050-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of MDCK using 13050-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 13050-1-AP (Claudin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HUVEC-Zellen, Maushautgewebe, Mauslebergewebe, Maus-Thymusgewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Hautkrebsgewebe, humanes malignes Melanomgewebe, humanes Ösophaguskarzinomgewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HUVEC-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 168 publications below
• IHC: See 36 publications below
• IF: See 33 publications below

Produktinformation

13050-1-AP bindet in WB, IHC, IF, ELISA Claudin 1 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Hund, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Claudin 1 fusion protein Ag3713
• Vollständiger Name: claudin 1
• Berechnetes Molekulargewicht: 211 aa, 23 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC012471
• Gene symbol: CLDN1
• Gene ID (NCBI): 9076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.

Publikationen

Species	Application	Title
mouse	IHC	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
human,mouse	IHC	Clin Cancer Res; ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism.; Authors - Mingyang Liu
human	IF	EMBO Rep; O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.; Authors - Ieva Bagdonaite
rat	IHC	Mater Today Bio; Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis; Authors - Jingzhe Wang
human	WB	PLoS Pathog; Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.; Authors - Suzuki Ryosuke R
rat	WB	Phytomedicine; Baizhu shaoyao decoction restores the intestinal barrier and brain-gut axis balance to alleviate diarrhea-predominant irritable bowel syndrome via FoxO1/FoxO3a; Authors - Yuanyuan Wei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (01-06-2022)

Works good. Nice image

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Gut

Claudin 1 Antibody Immunohistochemistry validation (1:100 dilution) in Gut (Cat no:13050-1-AP)

FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Keratinocytes

Claudin 1 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:13050-1-AP)

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