Claudin 11 Polyklonaler Antikörper

Claudin 11 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12152-1-AP

Synonyme

claudin 11, CLDN11, OSP, OTM

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Galerie der Validierungsdaten

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  WB analysis of human testis using 12152-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12152-1-AP (Claudin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12152-1-AP (Claudin 11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12152-1-AP (Claudin 11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12152-1-AP (Claudin 11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12152-1-AP (Claudin 11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12152-1-AP

  Immunofluorescent analysis of HepG2 cells, using CLDN11 antibody 12152-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IgG(red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 12152-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Claudin 11 antibody (12152-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

12152-1-AP bindet in WB, IHC, IF, FC, ELISA Claudin 11 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Claudin 11 fusion protein Ag2764
• Vollständiger Name: claudin 11
• Berechnetes Molekulargewicht: 207 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC013577
• Gene symbol: CLDN11
• Gene ID (NCBI): 5010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publikationen

Species	Application	Title
human	IF	Mol Cell Endocrinol; Cardiotonic steroid ouabain stimulates expression of blood-testis barrier proteins claudin-1 and -11 and formation of tight junctions in Sertoli cells.; Authors - Raimund Dietze
rat	WB	Environ Sci Pollut Res Int; Polystyrene microplastics induce blood-testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats.; Authors - Shengda Li
human	WB	J Physiol Biochem; α2,6-Sialylation promotes hepatocellular carcinoma cells migration and invasion via enhancement of nSmase2-mediated exosomal miRNA sorting; Authors - Liping Wang
human	WB	Sci Rep; Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis.; Authors - Zhaoxing Li

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