Claudin 18 Polyklonaler Antikörper

Claudin 18 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 21126-1-AP

Synonyme

claudin 18, CLDN18, SFTA5, SFTPJ

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 21126-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21126-1-AP (Claudin 18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse stomach using 21126-1-AP

  IP Result of anti-Claudin 18 (IP:21126-1-AP, 3ug; Detection:21126-1-AP 1:500) with mouse stomach tissue lysate 4000ug.

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  IHC staining of human stomach using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21126-1-AP (Claudin 18 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21126-1-AP (Claudin 18 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21126-1-AP (Claudin 18 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21126-1-AP (Claudin 18 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MKN-45 using 21126-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MKN-45 cells using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe
• Erfolgreiche IP: Maus-Magengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Magenkrebsgewebe, humanes Ovarialkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MKN-45-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

21126-1-AP bindet in WB, IP, IHC, IF, ELISA Claudin 18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Claudin 18 fusion protein Ag15517
• Vollständiger Name: claudin 18
• Berechnetes Molekulargewicht: 261 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28-29 kDa
• GenBank-Zugangsnummer: BC146668
• Gene symbol: CLDN18
• Gene ID (NCBI): 51208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145). This antibody can recognize both CLDN18.1 and CLDN18.2.

Publikationen

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
mouse	IHC	Ann Transl Med; Inhibition of protein kinase C alpha attenuates lipopolysaccharide-triggered acute lung injury by alleviating the hyperinflammatory response and oxidative stress.; Authors - Yang Chen
mouse	WB,IF	Int J Mol Med; Investigating the regulatory role of ORMDL3 in airway barrier dysfunction using in vivo and in vitro models.; Authors - Ruixue Yang
mouse	IF	BMC Pulm Med; SPAK-p38 MAPK signal pathway modulates claudin-18 and barrier function of alveolar epithelium after hyperoxic exposure.; Authors - Chih-Hao Shen
rat	IF	J Stroke Cerebrovasc Dis; Effect of MicroRNA-126a-3p on Bone Marrow Mesenchymal Stem Cells Repairing Blood-brain Barrier and Nerve Injury after Intracerebral Hemorrhage.; Authors - Chunyan Wang
human	IHC	Cancers (Basel); Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases; Authors - Maximiliano Gelli

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