Claudin 3 Polyklonaler Antikörper

Claudin 3 Polyklonal Antikörper für IHC, IP,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Rezensionen (2)

Kat-Nr. : 16456-1-AP

Synonyme

C7orf1, claudin 3, Claudin3, CLDN3, CPE R 2, CPE R2, CPE receptor 2, CPETR2, HRVP1, RVP1

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Galerie der Validierungsdaten

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  IP experiment of mouse large intestine using 16456-1-AP

  IP Result of anti-Claudin 3 (IP:16456-1-AP, 4ug; Detection:16456-1-AP 1:300) with mouse large intestine tissue lysate 3200ug.

• 

  IHC staining of human breast cancer using 16456-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16456-1-AP (Claudin 3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16456-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16456-1-AP (Claudin 3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 16456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16456-1-AP (Claudin 3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse colon using 16456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16456-1-AP (Claudin 3 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche IP: Maus-Dickdarmgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 8 publications below
• IF: See 9 publications below

Produktinformation

16456-1-AP bindet in IP, IHC, IF, ELISA Claudin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Claudin 3 fusion protein Ag9411
• Vollständiger Name: claudin 3
• Berechnetes Molekulargewicht: 220 aa, 23 kDa
• Beobachtetes Molekulargewicht: 20-24 kDa
• GenBank-Zugangsnummer: BC016056
• Gene symbol: CLDN3
• Gene ID (NCBI): 1365
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publikationen

Species	Application	Title
human	IHC	Aging Cell; Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts.; Authors - Zhi Chen
mouse	IF	EMBO Mol Med; Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.; Authors - Ming Zhao
mouse,rat	IHC	J Hazard Mater; Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.; Authors - Junrong Wu
mouse	IHC	Environ Int; Translocation of transition metal oxide nanoparticles to breast milk and offspring: The necessity of bridging mother-offspring-integration toxicological assessments.; Authors - Jie Cai
mouse		Biochem Pharmacol; Inhibition of S100A9 alleviates neurogenic pulmonary edema after subarachnoid hemorrhage; Authors - Guijun Wang
mouse	IHC	NanoImpact; Altered protein S-glutathionylation depicts redox imbalance triggered by transition metal oxide nanoparticles in a breastfeeding system.; Authors - Jie Cai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priya (Verified Customer) (07-31-2023)

Used for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

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