Claudin 3 Polyklonaler Antikörper

Claudin 3 Polyklonal Antikörper für IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(48)

Kat-Nr. : 16456-1-AP

Synonyme

C7orf1, claudin 3, Claudin3, CLDN3, CPE R 2, CPE R2, CPE receptor 2, CPETR2, HRVP1, RVP1



Geprüfte Anwendungen

Erfolgreiche IPMaus-Dickdarmgewebe
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, Maus-Kolongewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16456-1-AP bindet in IHC, IF, IP, ELISA Claudin 3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Claudin 3 fusion protein Ag9411
Vollständiger Name claudin 3
Berechnetes Molekulargewicht 220 aa, 23 kDa
Beobachtetes Molekulargewicht 20-24 kDa
GenBank-ZugangsnummerBC016056
Gene symbol Claudin 3
Gene ID (NCBI) 1365
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IHC protocol for Claudin 3 antibody 16456-1-APProtokoll herunterladenl
IP protocol for Claudin 3 antibody 16456-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIHC

J Hazard Mater

Insight into perfluorooctanoic acid-induced impairment of mouse embryo implantation via single-cell RNA-seq

Authors - Xiaoqiang Sheng
humanIHC

Aging Cell

Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts.

Authors - Zhi Chen
mouseIF

EMBO Mol Med

Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.

Authors - Ming Zhao
humanIF

Sci China Life Sci

Longitudinal gut fungal alterations and potential fungal biomarkers for the progression of primary liver disease

Authors - Shiman Jiang
mouse,ratIHC

J Hazard Mater

Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.

Authors - Junrong Wu
mouseIHC

Environ Int

Translocation of transition metal oxide nanoparticles to breast milk and offspring: The necessity of bridging mother-offspring-integration toxicological assessments.

Authors - Jie Cai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Priya (Verified Customer) (07-31-2023)

Used for Caco2 cells and mice tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon
FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon