CLEC12A Polyklonaler Antikörper

CLEC12A Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28627-1-AP

Synonyme

C-type lectin domain family 12 member A, CLL-1, CLL1, CLL 1, CD371

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  WB analysis of U-937 using 28627-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 28627-1-AP (CLEC12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28627-1-AP

  Human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 28627-1-AP (CLEC12A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of U-937 using 28627-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-937 cells without permeabilization using CLEC12A antibody (28627-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, human peripheral blood leukocyte
• Erfolgreiche Detektion in IF/ICC: U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28627-1-AP bindet in WB, IF/ICC, ELISA CLEC12A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLEC12A fusion protein Ag27864
• Vollständiger Name: C-type lectin domain family 12, member A
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 55-75 kDa
• GenBank-Zugangsnummer: BC063424
• Gene symbol: CLEC12A
• Gene ID (NCBI): 160364
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).