CLEC4G Polyklonaler Antikörper

CLEC4G Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18173-1-AP

Synonyme

CLEC4G, LP2698, LSECtin, UNQ431

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Galerie der Validierungsdaten

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  IHC staining of human liver using 18173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18173-1-AP (CLEC4G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 18173-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18173-1-AP (CLEC4G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 18173-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18173-1-AP (CLEC4G Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 18173-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18173-1-AP (CLEC4G Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver using 18173-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CLEC4G antibody (18173-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human liver using 18173-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CLEC4G antibody (18173-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Lebergewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18173-1-AP bindet in IHC, IF, ELISA CLEC4G und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLEC4G fusion protein Ag12837
• Vollständiger Name: C-type lectin domain family 4, member G
• Berechnetes Molekulargewicht: 293 aa, 33 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC093691
• Gene symbol: CLEC4G
• Gene ID (NCBI): 339390
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLEC4G, also known as LSECtin, is a member of the C-type lectin family of glycan-binding receptors that is expressed on sinusoidal endothelial cells in liver and lymph nodes. CLEC4G is a type II transmembrane protein of ∼40 kDa in size with a single C-type lectin-like domain at the COOH terminus, closest in homology to DC-SIGNR, DC-SIGN, and CD23. CLEC4G has been reported as a receptor for Japanese encephalitis virus (PubMed:24623090), ebolavirus (PubMed:16051304), SARS coronavirus/SARS-CoV (PubMed:16051304), lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed:22156524, PubMed:22673088).