CLIC4 Polyklonaler Antikörper

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• KD/KO Validated

CLIC4 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12298-2-AP

Synonyme

CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12298-2-AP

  WB result of CLIC4 antibody (12298-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells.

• 

  WB analysis of mouse kidney using 12298-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12298-2-AP (CLIC4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12298-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12298-2-AP (CLIC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 12298-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12298-2-AP (CLIC4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 12298-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12298-2-AP (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 12298-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12298-2-AP (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HeLa-Zellen, humanes Nierengewebe, Maus-Eierstockgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12298-2-AP bindet in WB, IHC,ELISA CLIC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLIC4 fusion protein Ag2942
• Vollständiger Name: chloride intracellular channel 4
• Berechnetes Molekulargewicht: 253 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC012444
• Gene symbol: CLIC4
• Gene ID (NCBI): 25932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.

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