CLP1 Polyklonaler Antikörper

CLP1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 14746-1-AP

Synonyme

CLP1, hClp1, HEAB, Polynucleotide kinase Clp1

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  WB analysis of mouse testis using 14746-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14746-1-AP (CLP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14746-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14746-1-AP (CLP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14746-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14746-1-AP (CLP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14746-1-AP bindet in WB, ELISA CLP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLP1 fusion protein Ag6479
• Vollständiger Name: CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 41 kDa, 48 kDa
• GenBank-Zugangsnummer: BC000446
• Gene symbol: CLP1
• Gene ID (NCBI): 10978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLP1, also named as HEAB, is a 425 amino acid protein, which belongs to the Clp1 family. CLP1 as a polynucleotide kinase, that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. Human CLP1 is a component of the mRNA 3'end cleavage and polyadenylation machinery4,5 and studies in yeast have postulated a function for CLP1 in coupling mRNA 3' end processing with RNA Pol II transcriptional termination. CLP1 exists 2 isoforms with MV 41 kDa and 48 kDa.

Publikationen

Species	Application	Title
human	WB	Mol Cell; m6Am sequesters PCF11 to suppress premature termination and drive neuroblastoma differentiation; Authors - Huihui An
		Nucleic Acids Res; Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.; Authors - Xiaodong Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (09-12-2025)

Consistently detects the target protein in WB. Tested on HeLa total cell extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

CLP1 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:14746-1-AP)