CLPP Polyklonaler Antikörper

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• KD/KO Validated

CLPP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(54)

Rezensionen (2)

Kat-Nr. : 15698-1-AP

Synonyme

Endopeptidase Clp, EC:3.4.21.92, ATP-dependent Clp protease proteolytic subunit, mitochondrial

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  WB analysis using 15698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15698-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 15698-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 15698-1-AP

  IP result of anti-CLPP (IP:15698-1-AP, 3ug; Detection:15698-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLPP antibody (15698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 15698-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15698-1-AP (CLPP antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, K-562-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 50 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

15698-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA CLPP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLPP fusion protein Ag8325
• Vollständiger Name: ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 26 kDa, 30 kDa
• GenBank-Zugangsnummer: BC002956
• Gene symbol: CLPP
• Gene ID (NCBI): 8192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.

Publikationen

Species	Application	Title
	WB	Cell Metab; Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.; Authors - Saara Forsström
mouse	WB	Cell Metab; mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.; Authors - Nahid A Khan
human	WB	Mol Cell; The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism; Authors - Wang Jiahui ; KD Validated
mouse	WB	Cell Metab; Proteolytic cleavage of opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation.; Authors - Prashant Mishra
mouse	WB	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anh (Verified Customer) (07-15-2021)

very good, very clear bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart cells

FH

Mariusz (Verified Customer) (02-06-2019)

Excellent antibody bot for Western blot (1:2000) dilution and immunofluorescence (1:200). Single band on Western, and clean signal reflecting mitochondrial matrix in IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:2000 (Western), 1:200 (IF)
• Cell Tissue Type: HeLa and HCt116