CLPP Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CLPP Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

2E1D9

Kat-Nr. : 66271-1-Ig

Synonyme

2E1D9, ATP-dependent Clp protease proteolytic subunit, mitochondrial, EC:3.4.21.92, Endopeptidase Clp

---

• 

  WB analysis of human heart using 66271-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66271-1-Ig (CLPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 66271-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66271-1-Ig (CLPP Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 66271-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66271-1-Ig (CLPP Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Hausschwein-Herzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

66271-1-Ig bindet in WB, IHC, IF, ELISA CLPP und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CLPP fusion protein Ag8420
• Vollständiger Name: ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 26 kDa, 30 kDa
• GenBank-Zugangsnummer: BC002956
• Gene symbol: CLPP
• Gene ID (NCBI): 8192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.

Publikationen

Species	Application	Title
human	WB	Nature; Enhancing mitochondrial proteostasis reduces amyloid-β proteotoxicity.; Authors - Vincenzo Sorrentino
human	IF	Cells; Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.; Authors - Jana Key
mouse	WB	PeerJ; Mitochondrial unfolded protein response gene CLPP changes mitochondrial dynamics and affects mitochondrial function.; Authors - GuiJun Wu ; KD Validated
human	WB	NeuroImmune Pharm Ther; Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages; Authors - Santosh Kumar
mouse	WB	Front Pharmacol; BAM8-22 targets spinal MrgC receptors to modulate UPRmt activity in the mechanism of bone cancer pain; Authors - Mingming Xie