Claspin Polyklonaler Antikörper

Claspin Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 23206-1-AP

Synonyme

hClaspin, CLSPN

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• 

  WB analysis of HEK-293 using 23206-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23206-1-AP (Claspin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 23206-1-AP

  IP result of anti-Claspin (IP:23206-1-AP, 5ug; Detection:23206-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

• 

  IHC staining of human stomach cancer using 23206-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23206-1-AP (Claspin antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 23206-1-AP

  Immunofluorescent analysis of HepG2 cells using 23206-1-AP (Claspin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HeLa using 23206-1-AP

  Immunofluorescent analysis of HeLa cells using 23206-1-AP (Claspin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

23206-1-AP bindet in WB, IF/ICC, IP, ELISA Claspin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Claspin fusion protein Ag19637
• Vollständiger Name: claspin homolog (Xenopus laevis)
• Berechnetes Molekulargewicht: 1339 aa, 151 kDa
• Beobachtetes Molekulargewicht: 240 kDa
• GenBank-Zugangsnummer: BC137279
• Gene symbol: Claspin
• Gene ID (NCBI): 63967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLSPN, also named as Claspin, is a 1339 amino acid protein, which belongs to the claspin family. CLSPN localizes in the nucleus and is highly expressed at S/G2 phases of the cell cycle. CLSPN is required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. CLSPN plays a potentially critical role in replication checkpoint control in cells and mediating phosphorylation and activation of checkpoint protein kinases. The calculated molecular weight of CLSPN is 151 kDa, but the modified protein is about 220-240 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Mol Life Sci; HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.; Authors - Uma Thanigai Arasu
human	WB	Int J Oncol; Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells.; Authors - Chao Wang
human	WB	Cell Insight; ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance; Authors - Rong Luo