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CLTA Polyklonaler Antikörper
CLTA Polyklonal Antikörper für IF, IHC, IP, WB,ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (1)
Anwendung
WB, IP, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10852-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Maushirngewebe, HEK-293-Zellen |
| Erfolgreiche IP | Maushirngewebe |
| Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | HepG2-Zellen, HEK-293-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF) | IF : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 9 publications below |
| IP | See 1 publications below |
Produktinformation
10852-1-AP bindet in WB, IP, IHC, IF, ELISA CLTA und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hund, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | CLTA fusion protein Ag1299 |
| Vollständiger Name | clathrin, light chain (Lca) |
| Berechnetes Molekulargewicht | 27 kDa |
| Beobachtetes Molekulargewicht | 35-38 kDa |
| GenBank-Zugangsnummer | BC019287 |
| Gene symbol | CLTA |
| Gene ID (NCBI) | 1211 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for CLTA antibody 10852-1-AP | Protokoll herunterladen |
| IHC protocol for CLTA antibody 10852-1-AP | Protokoll herunterladen |
| IF protocol for CLTA antibody 10852-1-AP | Protokoll herunterladen |
| IP protocol for CLTA antibody 10852-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Commun Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis. | ||
Proc Natl Acad Sci U S A Clathrin light chains' role in selective endocytosis influences antibody isotype switching.
| ||
J Cell Biol Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques. | ||
Small Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes. | ||
Acta Pharmacol Sin GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy | ||
J Bone Miner Res Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by CLTA Polyclonal antibody at dilution of 1:50 showed strong labelling.
|
FH K (Verified Customer) (06-10-2021) | this Ab works well for western blotting in mouse, human and rat cells
|
FH Cristian (Verified Customer) (04-17-2019) | Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)
 |
