• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CLTB Polyklonaler Antikörper

CLTB Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10455-1-AP

Synonyme

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of mouse thymus tissue using CLTB Polyclonal antibody (10455-1-AP)

    WB analysis of mouse thymus using 10455-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CLTB Polyclonal antibody (10455-1-AP)

    WB analysis of PC-3 using 10455-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CLTB Polyclonal antibody (10455-1-AP)

    WB analysis of mouse brain using 10455-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CLTB Polyclonal antibody (10455-1-AP)

    WB analysis of human brain using 10455-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CLTB Polyclonal antibody (10455-1-AP)

    IP experiment of mouse brain using 10455-1-AP

    IP Result of anti-CLTB (IP:10455-1-AP, 4ug; Detection:10455-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLTB Polyclonal antibody (10455-1-AP)

    IF Staining of HeLa using 10455-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CLTB antibody (10455-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CLTB Polyclonal antibody (10455-1-AP)

    IF Staining of HEK-293 using 10455-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CLTB antibody (10455-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaus-Thymusgewebe, humanes Hirngewebe, Maushirngewebe, PC-3-Zellen
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IFHeLa-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

KD/KOSee 1 publications below
WBSee 3 publications below

Produktinformation

10455-1-AP bindet in WB, IP, IF, ELISA CLTB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CLTB fusion protein Ag0707
Vollständiger Name clathrin, light chain (Lcb)
Berechnetes Molekulargewicht 23 kDa
Beobachtetes Molekulargewicht 32-38 kDa
GenBank-ZugangsnummerBC006457
Gene symbol CLTB
Gene ID (NCBI) 1212
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Protokolle

Produktspezifische Protokolle
WB protocol for CLTB antibody 10455-1-APProtokoll herunterladen
IF protocol for CLTB antibody 10455-1-APProtokoll herunterladen
IP protocol for CLTB antibody 10455-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Clathrin light chains' role in selective endocytosis influences antibody isotype switching.

Authors - Shuang Wu
  • KD Validated
View Article
mouseWB

Proc Natl Acad Sci U S A

Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo.

Authors - Lisa Redlingshöfer
View Article
humanWB

J Virol

Dabie bandavirus Nonstructural Protein Interacts with Actin to Induce F-Actin Rearrangement and Inhibit Viral Adsorption and Entry.

Authors - Hongyun Liu
View Article