CLTC Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CLTC Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

CloneNo.

1B3D7

Kat-Nr. : 66487-1-Ig

Synonyme

1B3D7, CHC, CHC17, Clathrin heavy chain, Clathrin heavy chain 1

---

• 

  WB analysis using 66487-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66487-1-Ig

  WB result of CLTC antibody (66487-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLTC transfected HeLa cells.

• 

  WB analysis using 66487-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66487-1-Ig

  HeLa, Jurkat, ROS1728 cells, and pig brain tissue were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66487-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 66487-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66487-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66487-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66487-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66487-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66487-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66487-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66487-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66487-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CLTC antibody (66487-1-Ig, Clone: 1B3D7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 66487-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66487-1-Ig (CLTC antibody), at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Raji-Zellen, Rattenhirngewebe, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Produktinformation

66487-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA CLTC und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CLTC fusion protein Ag25040
• Vollständiger Name: clathrin, heavy chain (Hc)
• Berechnetes Molekulargewicht: 192 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC054489
• Gene symbol: CLTC
• Gene ID (NCBI): 1213
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)

Publikationen

Species	Application	Title
human	WB	Front Immunol; Interactome of the Autoimmune Risk Protein ANKRD55.; Authors - Nerea Ugidos
pig	WB	Front Microbiol; Porcine Circovirus Type 3 Enters Into PK15 Cells Through Clathrin- and Dynamin-2-Mediated Endocytosis in a Rab5/Rab7 and pH-Dependent Fashion.; Authors - Ruihan Shi
mouse	IF	Cell Prolif; Perturbation of intestinal stem cell homeostasis and radiation enteritis recovery via dietary titanium dioxide nanoparticles; Authors - Linpei Zhang
	WB,IF	J Virol; Porcine Enteric Alphacoronavirus Entry through Multiple Pathways (Caveolae, Clathrin, and Macropinocytosis) Requires Rab GTPases for Endosomal Transport; Authors - Xiong-Nan Chen
human	WB	iScience; Flat clathrin lattices are linked to metastatic potential in colorectal cancer; Authors - Charlotte Cresens
mouse,human	WB,CoIP	Neuron; Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development; Authors - Minjie Shen