CMTM8 Polyklonaler Antikörper

CMTM8 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15039-1-AP

Synonyme

CKLFSF8, CKLFSF8 V2, CMTM8

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Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 15039-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15039-1-AP (CMTM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15039-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15039-1-AP (CMTM8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15039-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15039-1-AP (CMTM8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15039-1-AP

  IP Result of anti-CMTM8 (IP:15039-1-AP, 4ug; Detection:15039-1-AP 1:300) with mouse liver tissue lysate 6800ug.

• 

  IHC staining of human liver using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maus-Pankreasgewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Pankreasgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below

Produktinformation

15039-1-AP bindet in WB, IP, IHC, ELISA CMTM8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CMTM8 fusion protein Ag6776
• Vollständiger Name: CKLF-like MARVEL transmembrane domain containing 8
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC041390
• Gene symbol: CMTM8
• Gene ID (NCBI): 152189
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CMTM8, also known as CKLFSF8, is a member of the chemokine-like factor superfamily (CKLFSF). Proteins of this family share similarity with both the chemokine and the transmembrane 4 superfamilies. CMTM8 is an attenuator of EGF-induced signaling (PMID: 16263120). Overexpression of CMTM8 accelerates ligand-induced clearance of EGFR from the cell surface and induces apoptosis via caspase-dependent and -independent pathways (PMID: 17149703). An alternative splice form of CMTM8, CMTM8-v2, arises from a deletion of exon 2. CMTM8-v2 maintains the ability to induce apoptosis (PMID: 17681841).

Publikationen

Species	Application	Title
human	IHC	DNA Cell Biol; Downregulated CMTM8 Correlates with Poor Prognosis in Gastric Cancer Patients.; Authors - Meng Yan
human	IHC	BMC Gastroenterol; Identification and validation of EMT-immune-related prognostic biomarkers CDKN2A, CMTM8 and ILK in colon cancer.; Authors - Ning Kang
human	WB	Oncol Rep; CMTM8 inhibits the carcinogenesis and progression of bladder cancer.; Authors - Denghui Gao
human	WB	Bioengineered; MicroRNA-582-5p promotes triple-negative breast cancer invasion and metastasis by antagonizing CMTM8; Authors - Xue Zeng
	IHC	Cancers (Basel); The MAL Family of Proteins: Normal Function, Expression in Cancer, and Potential Use as Cancer Biomarkers; Authors - Leticia Labat-de-Hoz

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