CNN2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CNN2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 21073-1-AP

Synonyme

calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin

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  WB analysis of HeLa using 21073-1-AP

  WB result of CNN2 antibody (21073-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells.

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  WB analysis using 21073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21073-1-AP (CNN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hysteromyoma using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hysteromyoma using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21073-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CNN2 antibody (21073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21073-1-AP

  Immunofluorescent analysis of HepG2 cells, using CNN2 antibody 21073-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Uterusmyomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

21073-1-AP bindet in WB, IHC, IF/ICC, ELISA CNN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CNN2 fusion protein Ag15225
• Vollständiger Name: calponin 2
• Berechnetes Molekulargewicht: 309 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34-36 kDa
• GenBank-Zugangsnummer: BC141818
• Gene symbol: CNN2
• Gene ID (NCBI): 1265
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.

Publikationen

Species	Application	Title
mouse	IF	Circ Res; Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.; Authors - Felipe Paredes
human	IHC, WB	iScience; Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.; Authors - Holly Holliday
rat	WB	Front Pharmacol; Activation of the Cholinergic Anti-inflammatory Pathway Attenuated Angiotension II-Dependent Hypertension and Renal Injury.; Authors - Shu-Jie Wu
human	WB	Tumour Biol; Knockdown of calponin 2 suppressed cell growth in gastric cancer cells.; Authors - Jianwei Hu ; KD Validated
mouse	IF	Elife; Differential regulation of hair cell actin cytoskeleton mediated by SRF and MRTFB; Authors - Ling-Yun Zhou
mouse,rat	WB,IHC	Mol Metab; Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis; Authors - Yuan Gui ; KD Validated