CNPY2, MSAP Monoklonaler Antikörper

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• KD/KO Validated

CNPY2, MSAP Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G8G11

Kat-Nr. : 66173-1-Ig

Synonyme

canopy 2 homolog (zebrafish), CNPY2, CNPY2, MSAP, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

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Galerie der Validierungsdaten

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  WB analysis using 66173-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HepG2 using 66173-1-Ig

  WB result of CNPY2, MSAP antibody (66173-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells.

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  WB analysis of rat liver using 66173-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66173-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66173-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66173-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66173-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66173-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66173-1-Ig(CNPY2, MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-mouse IgG(H+L).

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  FC experiment of HepG2 using 66173-1-Ig

  1X10^6 HepG2 cells were stained with 0.20ug CNPY2, MSAP antibody (66173-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Hausschwein-Lebergewebe, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66173-1-Ig bindet in WB, IHC, IF, FC, ELISA CNPY2, MSAP und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CNPY2, MSAP fusion protein Ag6363
• Vollständiger Name: canopy 2 homolog (zebrafish)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 18-21 kDa
• GenBank-Zugangsnummer: BC065015
• Gene symbol: CNPY2
• Gene ID (NCBI): 10330
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2