COL6A3 Rekombinanter Antikörper

COL6A3 Rekombinant Antikörper für FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230540D3

Kat-Nr. : 83010-3-RR

Synonyme

COL6A3-Specific, 230540D3, Collagen alpha 3(VI) chain, Collagen alpha-3(VI) chain, Collagen Type VI

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  FC experiment of HeLa using 83010-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Col6a3-Specific Recombinant Antibody (83010-3-RR, Clone:230540D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83010-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83010-3-RR against Human COL6A3-Specific were performed. The affinity constant is 1.71 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83010-3-RR bindet in FC (Intra), ELISA COL6A3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: COL6A3 fusion protein Ag34752
• Vollständiger Name: collagen, type VI, alpha 3
• Berechnetes Molekulargewicht: 344 kDa
• Beobachtetes Molekulargewicht: 343 kDa
• GenBank-Zugangsnummer: NM_004369
• Gene symbol: COL6A3
• Gene ID (NCBI): 1293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.