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  • Featured Product
  • KD/KO Validated

COMMD1 Polyklonaler Antikörper

COMMD1 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-11938

Synonyme

C2orf5, COMMD1, MURR1, Protein Murr1

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated COMMD1 Polyclonal an (CL488-11938)

    IF Staining of HepG2 using CL488-11938

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 COMMD1 antibody (CL488-11938) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated COMMD1 Polyclonal an (CL488-11938)

    FC experiment of HepG2 using CL488-11938

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human COMMD1 (CL488-11938) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11938 bindet in IF, FC (Intra) COMMD1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen COMMD1 fusion protein Ag2535
Vollständiger Name copper metabolism (Murr1) domain containing 1
Berechnetes Molekulargewicht 190 aa, 21 kDa
Beobachtetes Molekulargewicht 21 kDa
GenBank-ZugangsnummerBC022046
Gene symbol COMMD1
Gene ID (NCBI) 150684
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-affinitätsgereinigt
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 COMMD1 antibody CL488-11938Protokoll herunterladen
FC protocol for CL Plus 488 COMMD1 antibody CL488-11938Protokoll herunterladen
Standard-Protokolle
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