COMMD1 Polyklonaler Antikörper

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• KD/KO Validated

COMMD1 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-11938

Synonyme

Protein Murr1, MURR1, COMM domain-containing protein 1, C2orf5

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  IF Staining of HepG2 using CL488-11938

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 COMMD1 antibody (CL488-11938) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-11938

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human COMMD1 (CL488-11938) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11938 bindet in IF/ICC, FC (Intra) COMMD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COMMD1 fusion protein Ag2535
• Vollständiger Name: copper metabolism (Murr1) domain containing 1
• Berechnetes Molekulargewicht: 190 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC022046
• Gene symbol: COMMD1
• Gene ID (NCBI): 150684
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.