COPG Polyklonaler Antikörper

COPG Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (3)

Kat-Nr. : 12393-1-AP

Synonyme

Gamma-1-COP, Gamma-1-coat protein, COPG1, Coatomer subunit gamma-1

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  WB analysis using 12393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12393-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 12393-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12393-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12393-1-AP

  IP result of anti-COPG (IP:12393-1-AP, 4ug; Detection:12393-1-AP 1:2000) with K-562 cells lysate 3600ug.

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  IHC staining of mouse kidney using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12393-1-AP (COPG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12393-1-AP (COPG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12393-1-AP (COPG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 12393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COPG antibody (12393-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 12393-1-AP

  Immunofluorescent analysis of HepG2 cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of NIH/3T3 using 12393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using COPG antibody (12393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 12393-1-AP

  Immunofluorescent analysis of Hela cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A375-Zellen, HeLa-Zellen, humanes Hirngewebe, humanes Kolongewebe, NIH/3T3-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

12393-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA COPG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COPG fusion protein Ag3070
• Vollständiger Name: coatomer protein complex, subunit gamma
• Berechnetes Molekulargewicht: 874 aa, 98 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC020498
• Gene symbol: COPG
• Gene ID (NCBI): 22820
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Deficiency in coatomer complex I causes aberrant activation of STING signalling.; Authors - Annemarie Steiner
	IF	J Exp Med; Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation.; Authors - Masahiko Nishitani-Isa
mouse	WB	J Biol Chem; Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice.; Authors - Xiaoling Li
mouse	WB	Hum Mol Genet; Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.; Authors - Desiree M Baron
human	IP,IF,WB	Sci Adv; HPV is a cargo for the COPI sorting complex during virus entry; Authors - Mara C Harwood
human	WB	Adv Sci (Weinh); Targeting COPA to Enhance Erdafitinib Sensitivity in FGFR-Altered Bladder Cancer; Authors - Huayuan Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

RICHA (Verified Customer) (03-02-2022)

Great antibody for IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse tendon fibroblast

FH

RICHA (Verified Customer) (11-16-2020)

Excellent antibody

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast

FH

RICHA (Verified Customer) (12-02-2019)

Excellent, no background

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast