COX16 Polyklonaler Antikörper

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• KD/KO Validated

COX16 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (1)

Kat-Nr. : 19425-1-AP

Synonyme

C14orf112, COX 16, hCOX16, HSPC203, MC4DN22

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  WB analysis using 19425-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19425-1-AP (COX16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19425-1-AP

  WB result of COX16 antibody (19425-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-COX16 transfected HeLa cells.

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  IHC staining of human ovary cancer using 19425-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19425-1-AP (COX16 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 19425-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19425-1-AP (COX16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 19425-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using COX16 antibody (19425-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below

Produktinformation

19425-1-AP bindet in WB, IHC, IF/ICC, ELISA COX16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX16 fusion protein Ag13756
• Vollständiger Name: COX16 cytochrome c oxidase assembly homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 106 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC001702
• Gene symbol: COX16
• Gene ID (NCBI): 51241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery; Authors - Jasmin Adriana Schafer
human	WB	Nat Commun; Coordination of metal center biogenesis in human cytochrome c oxidase.; Authors - Eva Nývltová
	WB	Nat Commun; The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium.; Authors - Chloé Maurizy
human	WB	Elife; COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis.; Authors - Abhishek Aich ; KO Validated
human	WB	J Biol Chem; An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.; Authors - Xiaofen Jin
mouse	WB	Redox Biol; Lead aggravates Alzheimer's disease pathology via mitochondrial copper accumulation regulated by COX17; Authors - Dingbang Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Süleyman (Verified Customer) (02-07-2022)

There are some unspecific bands which were distant but stronger than COX16 itself, even though I loaded 30-40ug of protein and 1.1000 dilution of antibody, either HeLa cells have very low abundant or antibody has some problems.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

COX16 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:19425-1-AP)