COX7A2L Polyklonaler Antikörper

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• KD/KO Validated

COX7A2L Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 11416-1-AP

Synonyme

COX7a related protein, COX7A2L, COX7AR, COX7RP, EB1, SIG81

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Galerie der Validierungsdaten

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  WB analysis using 11416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11416-1-AP (COX7A2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

11416-1-AP bindet in WB, IHC, IF, ELISA COX7A2L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX7A2L fusion protein Ag1980
• Vollständiger Name: cytochrome c oxidase subunit VIIa polypeptide 2 like
• Berechnetes Molekulargewicht: 114 aa, 13 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC007095
• Gene symbol: COX7A2L
• Gene ID (NCBI): 9167
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome c oxidase (COX) is the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. The mitochondrially encoded subunits function in electron transfer, and the nuclear encoded subunits may function in the regulation and assembly of the complex. COX7A2L (cytochrome c oxidase subunit 7A-related protein), also known as COX7AR or COX7RP, is an inner mitochondrial membrane protein. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. Recently it was found that DNA methylation of COX7A2L had a significant association with therapy response in patients with breast cancer. In Alzheimer's disease the expression of COX7A2L was reduced.

Publikationen

Species	Application	Title
mouse	WB	Science; Supercomplex assembly determines electron flux in the mitochondrial electron transport chain.; Authors - Esther Lapuente-Brun
human	WB	EMBO J; Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes.; Authors - Teresa Lobo-Jarne
human	WB	Cell Rep; Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics.; Authors - Teresa Lobo-Jarne ; KO Validated
		Free Radic Biol Med; Cardioprotective strategies preserve the stability of respiratory chain supercomplexes and reduce oxidative stress in reperfused ischemic hearts.; Authors - I Ramírez-Camacho
human	WB	Cell Rep; COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation.; Authors - Rafael Pérez-Pérez
human	WB,IHC,IF	J Pathol; Clinical response to chemotherapy in oesophageal adenocarcinoma patients is linked to defects in mitochondria.; Authors - Aichler Michaela M ; KD Validated