COXIV Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

COXIV Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

3K20

Kat-Nr. : 82916-1-RR

Synonyme

COX4I1, 3K20, COX IV-1, COX4, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

---

• 

  WB analysis of HepG2 using 82916-1-RR

  WB result of COXIV antibody (82916-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COXIV transfected HepG2 cells.

• 

  WB analysis using 82916-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 82916-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 82916-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 82916-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 82916-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovarian cancer using 82916-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82916-1-RR (COXIV antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 82916-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV antibody (82916-1-RR, Clone: 3K20 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 82916-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug COXIV Recombinant antibody (82916-1-RR, Clone:3K20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 82916-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82916-1-RR against Human COXIV were performed. The affinity constant is 9.41 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HepG2-Zellen, Jurkat-Zellen, Mauslebergewebe, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:600-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82916-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA COXIV und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: COXIV fusion protein Ag1640
• Vollständiger Name: cytochrome c oxidase subunit IV isoform 1
• Berechnetes Molekulargewicht: 19.6 kDa
• Beobachtetes Molekulargewicht: 17-18 kDa
• GenBank-Zugangsnummer: BC021236
• Gene symbol: COX IV
• Gene ID (NCBI): 1327
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues.