COXIV Monoklonaler Antikörper

COXIV Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(2)

CloneNo.

3C7D2

Kat-Nr. : CL488-60251

Synonyme

COX4I1, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX4, COX IV-1, 3C7D2

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  IF Staining of HepG2 using CL488-60251

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 COXIV antibody (CL488-60251, Clone: 3C7D2 ) at dilution of 1:200, CL594-phalloidin (red).

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  FC experiment of HeLa using CL488-60251

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human COXIV (CL488-60251, Clone:3C7D2) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

CL488-60251 bindet in IF/ICC, FC (Intra) COXIV und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: COXIV fusion protein Ag20551
• Vollständiger Name: cytochrome c oxidase subunit IV isoform 1
• Berechnetes Molekulargewicht: 19.6 kDa
• Beobachtetes Molekulargewicht: 17-18 kDa
• GenBank-Zugangsnummer: BC021236
• Gene symbol: COX IV
• Gene ID (NCBI): 1327
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.

Publikationen

Species	Application	Title
mouse	FC	Nat Immunol; Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis; Authors - Yavuz F Yazicioglu
mouse	IF	Life Sci; Short-term but not long-term high fat diet feeding protects against pressure overload-induced heart failure through activation of mitophagy.; Authors - Yanzhen Tan