Ceruloplasmin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Ceruloplasmin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 21131-1-AP

Synonyme

CP, CERU, Cuproxidase ceruloplasmin, EC:1.11.1.27, EC:1.11.1.9

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  WB analysis of human plasma using 21131-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 21131-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21131-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blutgewebe, humanes Plasmagewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolongewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

21131-1-AP bindet in WB, IHC, IF, ELISA Ceruloplasmin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ceruloplasmin fusion protein Ag15535
• Vollständiger Name: ceruloplasmin (ferroxidase)
• Berechnetes Molekulargewicht: 1065 aa, 122 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa, 85 kDa
• GenBank-Zugangsnummer: BC146663
• Gene symbol: Ceruloplasmin
• Gene ID (NCBI): 1356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Publikationen

Species	Application	Title
mouse,human	WB,IHC	Int J Biol Sci; Novel LncRNA LINC02936 Suppresses Ferroptosis and Promotes Tumor Progression by Interacting with SIX1/CP Axis in Endometrial Cancer; Authors - Zihui Zhang ; KD Validated
human	WB	Carcinogenesis; Identification of HCAR1 as a ferroptosis-related biomarker of gastric cancer based on a novel ferroptosis-related prognostic model and in vitro experiments; Authors - Hongjiao Zhang
chicken	IHC,IF	J Agric Food Chem; Drp1 Aggravates Copper Nanoparticle-Induced ER-Phagy by Disturbing Mitochondria-Associated Membranes in Chicken Hepatocytes; Authors - Quanwei Li
mouse	WB	J Neuroinflammation; Aberrant copper metabolism and hepatic inflammation cause neurological manifestations in a mouse model of Wilson's disease; Authors - Jianjian Dong
mouse	WB	Toxicology; Benzyl butyl phthalate promotes ferroptosis in Sertoli cells via disrupting ceruloplasmin-mediated iron balance; Authors - Yu Dong
human	WB,IHC	Cells; Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis; Authors - Shanshan Sha