Ceruloplasmin Polyklonaler Antikörper

Ceruloplasmin Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 21131-1-AP

Synonyme

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 21131-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 21131-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21131-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21131-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21131-1-AP (Ceruloplasmin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blutgewebe, humanes Plasmagewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21131-1-AP bindet in WB, IHC, IF, ELISA Ceruloplasmin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ceruloplasmin fusion protein Ag15535
• Vollständiger Name: ceruloplasmin (ferroxidase)
• Berechnetes Molekulargewicht: 1065 aa, 122 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa, 85 kDa
• GenBank-Zugangsnummer: BC146663
• Gene symbol: CP
• Gene ID (NCBI): 1356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Publikationen

Species	Application	Title
human	WB,IHC	Cells; Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis; Authors - Shanshan Sha
human	WB	J Clin Pharmacol; Expression of ceruloplasmin in the peripheral blood of patients with drug-resistant epilepsy; Authors - Hai-Yan Chen
mouse	WB,IF	Cell Death Dis; The divergent effects of astrocyte ceruloplasmin on learning and memory function in young and old mice; Authors - Zhong-Da Li
mouse	WB	iScience; Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activation; Authors - Jinsong Wei
mouse	WB	Mol Neurobiol; Gemfibrozil Alleviates Cognitive Impairment by Inhibiting Ferroptosis of Astrocytes via Restoring the Iron Metabolism and Promoting Antioxidant Capacity in Type 2 Diabetes; Authors - Nan Wang