Ceruloplasmin Monoklonaler Antikörper

Ceruloplasmin Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

3C8A5

Kat-Nr. : 66156-1-Ig

Synonyme

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

---

• 

  WB analysis of rat liver using 66156-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66156-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66156-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 66156-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 66156-1-Ig

  human blood was subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:50.

• 

  IHC staining of human liver using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:50.

• 

  IHC staining of human placenta using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66156-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ceruloplasmin antibody (66156-1-Ig, Clone: 3C8A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humanes Blutgewebe, humanes Nierengewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

66156-1-Ig bindet in WB, IHC, IF/ICC, ELISA Ceruloplasmin und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ceruloplasmin fusion protein Ag15535
• Vollständiger Name: ceruloplasmin (ferroxidase)
• Berechnetes Molekulargewicht: 1065 aa, 122 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC146663
• Gene symbol: Ceruloplasmin
• Gene ID (NCBI): 1356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest.; Authors - Marta Zarà
mouse	WB	J Proteomics; PMID: 31706944; Authors - Ni Yang
human	WB, IHC	FEBS Open Bio; Ceruloplasmin overexpression is associated with oncogenic pathways and poorer survival rates in clear-cell renal cell carcinoma.; Authors - Yong Zhang
human	WB	Clin Immunol; Proteomic profiling of aqueous humor-derived exosomes in Vogt-Koyanagi-Harada disease and Behcet's uveitis; Authors - Yinan Zhang
mouse	WB	Arch Toxicol; Arylacetamide deacetylase regulates hepatic iron homeostasis to protect against carbon tetrachloride-induced ferroptosis; Authors - Soshi Shinohara
human	WB	J Transl Med; The CD163 + tissue-infiltrating macrophages regulate ferroptosis in thyroid-associated ophthalmopathy orbital fibroblasts via the TGF-β/Smad2/3 signaling pathway; Authors - Xuemei Li