Carboxypeptidase A3 Polyklonaler Antikörper

Carboxypeptidase A3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16236-1-AP

Synonyme

CPA3, MC-CPA, MC CPA, Mast cell carboxypeptidase A, EC:3.4.17.1

---

• 

  WB analysis of mouse pancreas using 16236-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 16236-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 16236-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16236-1-AP (Carboxypeptidase A3 antibody at dilution of 1:50 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Pankreasgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

16236-1-AP bindet in WB, IHC, IF, ELISA Carboxypeptidase A3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Carboxypeptidase A3 fusion protein Ag8984
• Vollständiger Name: carboxypeptidase A3 (mast cell)
• Berechnetes Molekulargewicht: 417 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC012613
• Gene symbol: Carboxypeptidase A3
• Gene ID (NCBI): 1359
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Am J Pathol; Rare Pulmonary Connective Tissue Type Mast Cells Regulate Lung Endothelial Cell Angiogenesis.; Authors - Yue Ren
mouse	IF	Dev Cell; Mast cells proliferate in the peri-hippocampal space during early development and modulate local and peripheral immune cells; Authors - Alexa C Blanchard