CPEB1 Polyklonaler Antikörper

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• KD/KO Validated

CPEB1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 13274-1-AP

Synonyme

CEBP, CPE binding protein 1, CPE BP1, CPEB, CPEB1, h CEBP, hCPEB 1

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Galerie der Validierungsdaten

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  WB analysis using 13274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13274-1-AP (CPEB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13274-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 13274-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CPEB1 antibody (13274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

13274-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA CPEB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPEB1 fusion protein Ag3994
• Vollständiger Name: cytoplasmic polyadenylation element binding protein 1
• Berechnetes Molekulargewicht: 486 aa, 54 kDa
• Beobachtetes Molekulargewicht: 60-70kDa
• GenBank-Zugangsnummer: BC035348
• Gene symbol: CPEB1
• Gene ID (NCBI): 64506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPEB1, also named as CEBP, CPEB and CPE-BP1, contains two RRM (RNA recognition motif) domains and belongs to the RRM CPEB family. It is a sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. CPEB1 regulated translation is a key process involved in INS signaling. This is a rabbit polyclonal antibody raised against residues near the C terminus of human CPEB1.

Publikationen

Species	Application	Title
human,mouse	WB	Nature; Autism-like phenotype and risk gene mRNA deadenylation by CPEB4 mis-splicing.; Authors - Alberto Parras
human	WB,IF,IP	Nature; CPEB1 coordinates alternative 3'-UTR formation with translational regulation.; Authors - Felice-Alessio Bava ; KD Validated
mouse	WB	Nat Cell Biol; Ultrasensitive Ribo-seq reveals translational landscapes during mammalian oocyte-to-embryo transition and pre-implantation development.; Authors - Zhuqing Xiong
human,mouse,rat	WB,IHC,IP	Gastroenterology; Sequential Functions of CPEB1 and CPEB4 Regulate Pathologic Expression of Vascular Endothelial Growth Factor and Angiogenesis in Chronic Liver Disease.; Authors - Vittorio Calderone ; KD Validated
mouse	WB	Adv Sci (Weinh); Selective Translation of Maternal mRNA by eIF4E1B Controls Oocyte to Embryo Transition; Authors - Jing Guo
human	WB	Nat Commun; Translational reprogramming in tumour cells can generate oncoselectivity in viral therapies.; Authors - Eneko Villanueva

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