CPNE3 Polyklonaler Antikörper

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• KD/KO Validated

CPNE3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 11186-1-AP

Synonyme

Copine 3, Copine III, Copine-3, copineIII, CPN3

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  WB analysis using 11186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11186-1-AP (CPNE3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11186-1-AP

  WB result of CPNE3 antibody (11186-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPNE3 transfected A431 cells.

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  IP experiment of HepG2 using 11186-1-AP

  IP result of anti-CPNE3 (IP:11186-1-AP, 4ug; Detection:11186-1-AP 1:10000) with HepG2 cells lysate 1320 ug.

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  IHC staining of mouse liver using 11186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11186-1-AP (CPNE3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 11186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11186-1-AP (CPNE3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11186-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CPNE3 antibody (11186-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, HeLa-Zellen, HepG2-Zellen, HuH-7-Zellen, Y79-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below

Produktinformation

11186-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CPNE3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPNE3 fusion protein Ag1634
• Vollständiger Name: copine III
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC036242
• Gene symbol: CPNE3
• Gene ID (NCBI): 8895
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.

Publikationen

Species	Application	Title
human	WB,IHC	J Cell Physiol; Circulating exosomal CPNE3 as a diagnostic and prognostic biomarker for colorectal cancer.; Authors - Bo Sun
human	IHC	Cell Mol Life Sci; YAP1-CPNE3 positive feedback pathway promotes gastric cancer cell progression; Authors - Xuan Li
mouse	WB	Ecotoxicol Environ Saf; Polystyrene nanoplastics accelerate atherosclerosis: Unraveling the impact on smooth muscle cells through KIF15-mediated migration; Authors - Yizhou Zhong
human	WB,IHC	Front Immunol; Integration of transcriptomics and machine learning for insights into breast cancer: exploring lipid metabolism and immune interactions; Authors - Xiaohan Chen