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CPNE4 Polyklonaler Antikörper

CPNE4 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16075-1-AP

Synonyme

Copine 4, Copine 8, copine IV, COPN4, CPN4, CPNE4

Filter:
  • Western Blot (WB) analysis of various lysates using CPNE4 Polyclonal antibody (16075-1-AP)

    WB analysis using 16075-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16075-1-AP (CPNE4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CPNE4 Polyclonal antibody (16075-1-AP)

    IF Staining of A549 using 16075-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CPNE4 antibody (16075-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IF/ICCA549-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16075-1-AP bindet in WB, IF/ICC, ELISA CPNE4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CPNE4 fusion protein Ag9037
Vollständiger Name copine IV
Berechnetes Molekulargewicht 557 aa, 62 kDa
Beobachtetes Molekulargewicht62 kDa
GenBank-ZugangsnummerBC014396
Gene symbol CPNE4
Gene ID (NCBI) 131034
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions. CPNE4 (Copine 4) belongs to the copine family. This antibody is specific to CPNE4.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CPNE4 antibody 16075-1-APProtokoll herunterladen
IF protocol for CPNE4 antibody 16075-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Cajsa (Verified Customer) (09-17-2024)

Great antibody. Image of enteric neuronal nuclei (green, Baf53 reporter) and Cpna4 (red).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Myenteric plexus of adult mouse intestine
CPNE4 Antibody Immunofluorescence validation (1:300 dilution) in Myenteric plexus of adult mouse intestine (Cat no:16075-1-AP)