INTS11 Polyklonaler Antikörper

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• KD/KO Validated

INTS11 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 15860-1-AP

Synonyme

INT11, Cleavage and polyadenylation-specific factor 3-like protein, CPSF3L, CPSF3-like protein, EC:3.1.27.-

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  WB analysis of mouse colon using 15860-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15860-1-AP (INTS11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 15860-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15860-1-AP (INTS11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 15860-1-AP

  Immunofluorescent analysis of HepG2 cells using 15860-1-AP (INTS11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

15860-1-AP bindet in WB, IF/ICC, ELISA INTS11 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INTS11 fusion protein Ag8365
• Vollständiger Name: cleavage and polyadenylation specific factor 3-like
• Berechnetes Molekulargewicht: 600 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC007978
• Gene symbol: INT11
• Gene ID (NCBI): 54973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrator complex subunit 11 (INT11) is a protein that in humans is encoded by the INTS11 gene. INTS11, also known as Cleavage and Polyadenylation Specific Factor 3-Like (CPSF3L), contains a metallo-β-lactamase and β-CASP domain that, together, coordinate two zinc ions within the active site to promote its RNA endonuclease activity. INTS11 is the catalytic subunit of the Integrator (INT) complex, which is crucial for the biogenesis of small nuclear RNAs and enhancer RNAs. INT11 has 5 isoforms with the molecular mass of 56 and 65-68 kDa (PMID: 34516911, PMID: 37054711).

Publikationen

Species	Application	Title
human	WB	Open Med (Wars); Comprehensive landscape of integrator complex subunits and their association with prognosis and tumor microenvironment in gastric cancer; Authors - Xiaoxia Tong ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Britt (Verified Customer) (05-03-2023)

Antibody works well and is specific to target. Got a nice clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HuH7