CPT2 Polyklonaler Antikörper

CPT2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Rezensionen (1)

Kat-Nr. : 26555-1-AP

Synonyme

CPT II, CPT1, CPT2, CPTASE

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Galerie der Validierungsdaten

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  WB analysis using 26555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26555-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26555-1-AP (CPT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 26555-1-AP

  IP Result of anti-CPT2 (IP:26555-1-AP, 4ug; Detection:26555-1-AP 1:300) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26555-1-AP (CPT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C2C12 using 26555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using 26555-1-AP (CPT2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, LNCaP-Zellen, Mausherzgewebe, Mauslebergewebe, MCF-7-Zellen, Rattenlebergewebe, T-47D-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 4 publications below

Produktinformation

26555-1-AP bindet in WB, IP, IHC, IF, ELISA CPT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPT2 fusion protein Ag24897
• Vollständiger Name: carnitine palmitoyltransferase 2
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC002445
• Gene symbol: CPT2
• Gene ID (NCBI): 1376
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB,IP	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
mouse	WB	Nat Commun; Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling; Authors - Jing Zhong
mouse	WB	Theranostics; Mitochondrial STAT3 exacerbates LPS-induced sepsis by driving CPT1a-mediated fatty acid oxidation.; Authors - Rongqing Li
mouse	WB,IP	Elife; Remodeling of whole-body lipid metabolism and a diabetic-like phenotype caused by loss of CDK1 and hepatocyte division.; Authors - Jin Rong Ow
mouse	WB	J Nanobiotechnology; Regulating immune memory and reversing tumor thermotolerance through a step-by-step starving-photothermal therapy; Authors - Lihua Luo
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mitchell (Verified Customer) (09-26-2019)

Worked great for western blot in proximal intestine and liver.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Intestine and Liver