CRABP1 Polyklonaler Antikörper

CRABP1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12588-1-AP

Synonyme

CRABP, CRABP I, CRABP1, CRABPI, RBP5

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Galerie der Validierungsdaten

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  WB analysis of mouse embryo using 12588-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 12588-1-AP (CRABP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 12588-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12588-1-AP (CRABP1 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 12588-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12588-1-AP (CRABP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 12588-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12588-1-AP (CRABP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12588-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12588-1-AP (CRABP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausembryogewebe, humanes Milzgewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12588-1-AP bindet in WB, IHC, IF, ELISA CRABP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRABP1 fusion protein Ag3291
• Vollständiger Name: cellular retinoic acid binding protein 1
• Berechnetes Molekulargewicht: 137 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC022069
• Gene symbol: CRABP1
• Gene ID (NCBI): 1381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cellular retinoic acid-binding proteins including CRABP1 and CRABP2 bind retinoic acid (RA), an important regulator of cell growth and differentiation, thus play an important role in RA-mediated differentiation and proliferation processes. It has been reported that CRABP1 influences the biological effects of RA in cell-selective manners by enhancing the physiological function of RA in keratinocytes or inhibiting RA-induced differentiation of neuroblastoma cells.

Publikationen

Species	Application	Title
chicken	IF	Elife; Single-cell proteomics reveals changes in expression during hair-cell development.; Authors - Ying Zhu
human	WB,IHC	Mol Oncol; CRABP-II methylation: a critical determinant of retinoic acid resistance of medulloblastoma cells.; Authors - Fu Yuan-Shan YS
human	WB	Cell Rep; Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.; Authors - Marie Kubota
mouse	WB	Virol J; All-trans retinoic acid increases the pathogenicity of the H9N2 influenza virus in mice; Authors - Xiaofei Niu
		Cell Rep; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration; Authors - Lixiang Sun