CREB1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CREB1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(171)

Rezensionen (2)

Kat-Nr. : 12208-1-AP

Synonyme

cAMP-responsive element-binding protein 1, CREB, CREB 1, CREB-1, Cyclic AMP-responsive element-binding protein 1

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  WB analysis using 12208-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12208-1-AP

  WB result of CREB1 antibody (12208-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB1 transfected HEK-293 cells.

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  WB analysis using 12208-1-AP

  WB result of 12208-1-AP (CREB1 antibody) with various lysates at dilution of 1:1,500.

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  WB analysis of HL-1 using 12208-1-AP

  N/A

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  WB analysis of mouse brain using 12208-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12208-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12208-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 12208-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12208-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12208-1-AP

  IP result of anti-CREB1 (IP:12208-1-AP, 4ug; Detection:12208-1-AP 1:2000) with HEK-293 cells lysate 1360 ug.

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  IHC staining of mouse brain using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human thyroid using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 12208-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12208-1-AP (CREB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 12208-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREB1 antibody (12208-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 12208-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREB1 antibody (12208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 12208-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CREB1 antibody (12208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 12208-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human CREB1 (12208-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  ChIP experiment of HEK-293 using 12208-1-AP

  Chromatin was prepared from HEK-293 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 µg of cross-linked chromatin, 5 µg of CREB1 Antibody (12208-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, COS-7-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, Maushirngewebe, Mauslungengewebe, Multizellen/-gewebe, NIH/3T3-Zellen, PC-12-Zellen, PC-3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Zervixkarzinomgewebe, humanes Prostatakarzinomgewebe, humanes Schilddrüsengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen
• Erfolgreiche ChIP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHImmunpräzipitation (IP): CHIP : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 159 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 8 publications below
• RIP: See 1 publications below

Produktinformation

12208-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA CREB1 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CREB1 fusion protein Ag2852
• Vollständiger Name: cAMP responsive element binding protein 1
• Berechnetes Molekulargewicht: 341 aa, 35 kDa
• Beobachtetes Molekulargewicht: 43-46 kDa
• GenBank-Zugangsnummer: BC010636
• Gene symbol: CREB1
• Gene ID (NCBI): 1385
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).

Publikationen

Species	Application	Title
mouse,human	WB	Cell Metab; Lactate modulates iron metabolism by binding soluble adenylyl cyclase; Authors - Wei Liu
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
mouse	WB	Mol Cell; LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation; Authors - Shelby E Compton
human	WB	Mol Cancer; CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.; Authors - Bing Yao
mouse,human	WB	Environ Int; Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms; Authors - Chaoyang Liu
mouse	WB	Redox Biol; Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke; Authors - Haiyun Guo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Georgiana (Verified Customer) (01-10-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain tissue, cortex, 20um section

CREB1 Antibody Immunofluorescence validation (1:500 dilution) in Mouse brain tissue, cortex, 20um section (Cat no:12208-1-AP)

FH

Shogo (Verified Customer) (02-22-2019)

Approximately 50 mg of whole brain were applied to ChIP assay.Fragmented DNA was immunoprecipitated with the CREB antibody (250 ng) at 4 oC for overnight.

• Applications: ChIP,
• Cell Tissue Type: Brain