CREB3 Polyklonaler Antikörper

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• KD/KO Validated

CREB3 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 11275-1-AP

Synonyme

CREB 3, CREB3, LUMAN, LZIP

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11275-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11275-1-AP (CREB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11275-1-AP

  WB result of CREB3 antibody (11275-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB3 transfected HeLa cells.

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  IHC staining of human prostate cancer using 11275-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11275-1-AP (CREB3 antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of PC-3 using 11275-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 11275-1-AP (CREB3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 2 publications below

Produktinformation

11275-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA CREB3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CREB3 fusion protein Ag1823
• Vollständiger Name: cAMP responsive element binding protein 3
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 40-64 kDa
• GenBank-Zugangsnummer: BC009402
• Gene symbol: CREB3
• Gene ID (NCBI): 10488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).

Publikationen

Species	Application	Title
human	WB	Sci Adv; Standalone or combinatorial phenylbutyrate therapy shows excellent antiviral activity and mimics CREB3 silencing.; Authors - Tejabhiram Yadavalli ; KD Validated
human	ChIP	Nat Commun; Golgi stress induces SIRT2 to counteract Shigella infection via defatty-acylation.; Authors - Miao Wang
human	WB	Int J Mol Sci; Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells.; Authors - Kentaro Oh-Hashi
human	WB,IHC	Cancers (Basel); miR766-3p and miR124-3p Dictate Drug Resistance and Clinical Outcome in HNSCC; Authors - Tomohiro Shibata
human	WB,IF,IP,ChIP	Front Mol Neurosci; LUMAN/CREB3 Plays a Dual Role in Stress Responses as a Cofactor of the Glucocorticoid Receptor and a Regulator of Secretion.; Authors - Jenna Penney
human		Carcinogenesis; Small leucine zipper protein promotes the metastasis of castration-resistant prostate cancer through transcriptional regulation of matrix metalloproteinase-13.; Authors - Suhyun Kim