CREM Polyklonaler Antikörper

CREM Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 12131-1-AP

Synonyme

cAMP-responsive element modulator, hCREM 2, ICER

---

• 

  WB analysis using 12131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12131-1-AP (CREM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 12131-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12131-1-AP (CREM Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12131-1-AP

  IP result of anti-CREM (IP:12131-1-AP, 4ug; Detection:12131-1-AP 1:300) with mouse testis tissue lysate 1800ug.

• 

  IHC staining of human prostate cancer using 12131-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12131-1-AP (CREM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12131-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12131-1-AP (CREM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhodengewebe, Maus-Thymusgewebe, Ratten-Thymusgewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

12131-1-AP bindet in WB, IHC, IF, IP, ELISA CREM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CREM fusion protein Ag2775
• Vollständiger Name: cAMP responsive element modulator
• Berechnetes Molekulargewicht: 332 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC017117
• Gene symbol: CREM
• Gene ID (NCBI): 1390
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclic AMP-responsive element modulator (CREM) is a transcription factor highly expressed in the post-meiotic germ cells of the testis. Its pivotal role is to regulate the expression of several germ cell-specific genes, a crucial function as demonstrated by the severe phenotype of mice whose CREM gene was mutated by homologous recombination. CREM-deficient male animals are sterile and display a ten-fold increase in the apoptosis of germ cells. Recent results have shown that CREM needs a tissue-specific co-activator, ACT (activator of CREM in testis) to elicit its regulatory function in testis (PMID: 10824972). Also, CREM is a crucial regulator of NK cell function. CREM exerts its regulatory functions through epigenetic reprogramming of CAR-NK cells (PMID: 40468083).

Publikationen

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
mouse	IF	Biofabrication; Testis on a chip - a microfluidic 3-dimensional culture system for the development of spermatogenesisin-vitro.; Authors - Ali AbuMadighem
mouse	IF	Int J Mol Sci; Effect of Chemotherapy Cytarabine and Acute Myeloid Leukemia on the Development of Spermatogenesis at the Adult Age of Immature Treated Mice.; Authors - Bara'ah Khaleel
mouse	IF	Int J Mol Sci; The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells.; Authors - Noy Bagdadi
mouse	IF	Int J Mol Sci; Effect of Temperature on the Development of Stages of Spermatogenesis and the Functionality of Sertoli Cells In Vitro; Authors - Areej Jorban
	IF	Life Sci; Adrenocorticotropic hormone and its receptor as a novel testicular system involves in the development of spermatogenesis; Authors - AbuMadighem Ali